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Chitosan/gelatin porous scaffolds assembled with the conductive poly(3,4-ethylenedioxythiophene) nanoparticles for neural tissue engineering

Abstract

Electroactive biomaterials are widely explored as scaffolds for nerve tissue regeneration. Poly (3,4-ethylenedioxythiophene) (PEDOT), as a conductive polymer, has been chosen to construct tissue engineered scaffold because of its excellent conductivity and non-cytotoxicity. In the present work, an electrically conductive scaffold was prepared by assembling PEDOT on chitosan/gelatin (Cs/Gel) porous scaffolds surface via in situ interfacial polymerization. The hydrophilic Cs/Gel hydrogel was used as a template and PEDOT nanoparticles were uniformly assembled on the scaffold surface. The static polymerization of 3,4-ethylenedioxythiophene (EDOT) monomer in the interface between an aqueous phase and the organic phase was accompanied with the formation of PEDOT-assembled Cs/Gel scaffolds. PEDOT/Cs/Gel scaffolds were characterized by scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The results confirmed the deposition of PEDOT nanoparticles with mean diameter of 50 nm on the Cs/Gel scaffold channel surface. Compared with the Cs/Gel scaffold, the incorporation of PEDOT on scaffold increased the electrical conductivity, hydrophilicity, mechanical properties and thermal stability while decreasing the water absorption and biodegradation. For the biocompatibility, PEDOT/Cs/Gel scaffolds, especially 2PEDOT/Cs/Gel scaffold group significantly promoted neuron-like rat phaeochromocytoma (PC12) cells adhesion and proliferation. The results of both the gene expression and protein level assessments suggested that the PEDOT-assembled Cs/Gel scaffold enhanced the PC12 cellular neurite growth with higher protein and gene expression levels. This is the first report on the construction of conductive PEDOT/Cs/Gel porous scaffold via in situ interfacial polymerization method and the results demonstrate it may be a promising conductive scaffold for neural tissue engineering.

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Publication details

The article was received on 05 Mar 2017, accepted on 13 May 2017 and first published on 15 May 2017


Article type: Paper
DOI: 10.1039/C7TB00608J
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    Chitosan/gelatin porous scaffolds assembled with the conductive poly(3,4-ethylenedioxythiophene) nanoparticles for neural tissue engineering

    S. Wang, C. Sun, S. Guan, W. Li, J. Xu, D. Ge, M. Zhuang, T. Liu and X. Ma, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C7TB00608J

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