A bio-inspired high strength three-layer nanofibers vascular graft with structural guided cells growth
Natural blood vessels of human are of three-layer structures including tunica intima, tunica media, and tunica adventitia. These subtle structures enable health blood vessels outstanding strength, elasticity, compliance as well as excellent haemodynamic and anti-thrombus performance. Fabrication of next generation vascular graft that mimics the structures and functionalities of natural blood vessels is becoming the pursuit of biomaterial and medical scientists. Here we fabricate a bio-inspired nanofibers three-layer vascular graft by electrospinning. The inner PLA/PCL layer is favorable adhesion of human umbilical vein endothelial cells that could accelerate endothelialization. The middle PU/PCL layer provides superior mechanical properties (63.40 MPa, 266.78% in longitudinal direction and 52.34 MPa, 319.72% in lateral direction). The outer PLA/PCL layer with circumferential aligned fibers is beneficial for guiding vascular smooth muscle cells in circumferential oriented direction. The bio-inspired three-layer vascular graft with strong mechanical properties and good cell biocompatibility will play an important role in vessel remodeling and regeneration.