Jump to main content
Jump to site search

Issue 16, 2017
Previous Article Next Article

Dual delivery of siRNA and plasmid DNA using mesoporous silica nanoparticles to differentiate induced pluripotent stem cells into dopaminergic neurons

Author affiliations

Abstract

The cells of the central nervous system (CNS) show irreversible features after injury, and they could be re-established by stem cells. Induced pluripotent stem cells (iPSCs) may be generated from adult mammalian cells and gain pluripotency to differentiate into various lineages and functional cells. Non-viral carriers for delivering differentiation factors to transform iPSCs into neuronal cells are very desirable. In this study, mesoporous silica nanoparticles (MSNs) are used to co-deliver Nurr1 plasmid DNA (pNurr1) and Rex1 siRNA (siRex1) into iPSCs to achieve dopaminergic neuron differentiation. Sixty hours after treatment with siRex1 and pNurr1, the co-delivery of pNurr1 and siRex1 enhanced the Nurr1 gene expression three-fold, compared to the delivery of the plasmid pNurr1 only. The dopaminergic neuron-related protein level was identified using immunofluorescence staining and flow cytometry analysis, and there were 89.9% ± 0.5% tyrosine hydroxylase-expressing cells and 88.5% ± 2.0% dopamine transporter-expressing cells differentiated from iPSCs after transfection by MSNs. An ELISA was also used to determine the maturation of functional neurons, and there was 12.19 ng mL−1 dopamine released from cells after transfection by MSNs. These results demonstrate that MSNs are good non-viral nanocarriers for dual delivery of pNurr1 and siRex1 to significantly enhance the generation of dopaminergic neurons from iPSCs.

Graphical abstract: Dual delivery of siRNA and plasmid DNA using mesoporous silica nanoparticles to differentiate induced pluripotent stem cells into dopaminergic neurons

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Feb 2017, accepted on 17 Mar 2017 and first published on 17 Mar 2017


Article type: Paper
DOI: 10.1039/C7TB00351J
Citation: J. Mater. Chem. B, 2017,5, 3012-3023
  •   Request permissions

    Dual delivery of siRNA and plasmid DNA using mesoporous silica nanoparticles to differentiate induced pluripotent stem cells into dopaminergic neurons

    J. Chang, P. Tsai, W. Chen, S. Chiou and C. Mou, J. Mater. Chem. B, 2017, 5, 3012
    DOI: 10.1039/C7TB00351J

Search articles by author

Spotlight

Advertisements