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Issue 12, 2017
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A visible-light-excited Eu3+ complex-based luminescent probe for highly sensitive time-gated luminescence imaging detection of intracellular peroxynitrite

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Abstract

Although considerable efforts have been made for the development of lanthanide complex-based luminescent probes, a major drawback of the current probes is the requirement for UV excitation. In this work, a visible-light-excited Eu3+ complex-based luminescent probe, [Eu(HDPH)3(DPBT)] (HDPH: 1,1,1,2,2,3,3-heptafluoro-6-(2,4-dimethoxyphenyl)-4,6-hexanedione; DPBT: 2-(N,N-diethylanilin-4-yl)-4,6-bis(3,5-dimethylpyrazol-1-yl)-1,3,5-triazine), has been designed and synthesized for the highly sensitive and selective time-gated luminescence imaging detection of peroxynitrite (ONOO) in living cells. The probe exhibits a strong visible excitation peak at 400 nm, with the excitation window extending to beyond 460 nm. Upon reaction with ONOO, the luminescence of the probe at 610 nm is greatly quenched because the excited state of the Eu3+ complex is deactivated by the interaction between the 2,4-dimethoxyphenyl moiety and ONOO. The dose-dependent luminescence decrease of the probe shows good linearity with a detection limit of 1.8 μM for ONOO, and the probe can specifically distinguish ONOO from other reactive oxygen/nitrogen species (ROS/RNS). The [Eu(HDPH)3(DPBT)]-loaded HepG2 cells were prepared, and then the exogenous ONOO in HepG2 cells was successfully imaged in time-gated luminescence mode. The photostability results of the [Eu(HDPH)3(DPBT)]-loaded HepG2 cells demonstrate the practical applicability of the visible-light-excited probes for long-term cell imaging.

Graphical abstract: A visible-light-excited Eu3+ complex-based luminescent probe for highly sensitive time-gated luminescence imaging detection of intracellular peroxynitrite

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Publication details

The article was received on 03 Feb 2017, accepted on 28 Feb 2017 and first published on 13 Mar 2017


Article type: Paper
DOI: 10.1039/C7TB00345E
Citation: J. Mater. Chem. B, 2017,5, 2322-2329
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    A visible-light-excited Eu3+ complex-based luminescent probe for highly sensitive time-gated luminescence imaging detection of intracellular peroxynitrite

    J. Wu, Y. Yang, L. Zhang, H. Wang, M. Yang and J. Yuan, J. Mater. Chem. B, 2017, 5, 2322
    DOI: 10.1039/C7TB00345E

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