Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Amino acid-modified chitosan nanoparticles for Cu2+ chelation to suppress CuO nanoparticle cytotoxicity

Author affiliations

Abstract

The extensive development and application of engineered nanoparticles (NPs) in various fields worldwide have been subjected to increasing concern due to their potential hazards to human health and the environment. Therefore, a simple, economical, and effective method for suppressing the toxicity of metal-based nanomaterials is needed. In this study, glutaraldehyde-crosslinked chitosan nanoparticles (CS NPs) were prepared and further modified with lysine (Ly-CS), glutamic acid (Glu-CS), or sodium borohydride reduction (R-CS), and used to suppress cytotoxicity induced by copper oxide NPs (CuO NPs) through chelation with intracellularly released copper ions. All three kinds of CS NPs had similar sizes of ∼100 nm in a dry state and ∼200 nm in cell culture medium, as determined by scanning electron microscopy, transmission electron microscopy, and dynamic light scattering. The chelating efficiency of different CS NPs followed the order Ly-CS > Glu-CS > R-CS. The CS NPs showed minimal or no toxicity to three different cell lines (HepG2, A549, and RAW264.7 cells) at 100 μg mL−1 with similar cell internalization and exocytosis processes. Comparatively, RAW264.7 cells exhibited higher endocytosis and exocytosis rates, as revealed by flow cytometry and confocal laser scanning microscopy. CS NPs were found as agglomerates inside A549 cells and RAW264.7 cells, with the amount of agglomerates inside RAW264.7 cells decreasing significantly with prolonged incubation. All three CS NPs, especially Ly-CS and Glu-CS NPs, efficiently suppressed the cytotoxicity induced by CuO NPs, and reduced the intracellular level of reactive oxygen species.

Graphical abstract: Amino acid-modified chitosan nanoparticles for Cu2+ chelation to suppress CuO nanoparticle cytotoxicity

Back to tab navigation
Please wait while Download options loads

Publication details

The article was received on 03 Feb 2017, accepted on 09 Apr 2017 and first published on 10 Apr 2017


Article type: Paper
DOI: 10.1039/C7TB00344G
Citation: J. Mater. Chem. B, 2017, Advance Article
  •   Request permissions

    Amino acid-modified chitosan nanoparticles for Cu2+ chelation to suppress CuO nanoparticle cytotoxicity

    Y. Zhang, Y. Xu, X. Xi, S. Shrestha, P. Jiang, W. Zhang and C. Gao, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C7TB00344G

Search articles by author