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Issue 18, 2017
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Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots

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Abstract

Within living cells, the biological functions of subcellular organelles are highly dependent on the distribution of local temperature. In this regard, sensitive and accurate measurement of cellular temperature with subcellular resolution is fundamentally and practically significant. In this work, we demonstrated a fluorescence-based self-calibration method for the quantitative analysis of living cell temperature. A ratiometric temperature sensor was designed by conjugating a temperature sensitive dye, Rhodamine B (RhB), with biocompatible carbon dots (CDs) via a covalent bond. The as-prepared CDs–RhB probe showed excellent ratiometric temperature sensing capability in solution under a single wavelength excitation and displayed a linear temperature sensing range from 5 to 50 °C, which matches the requirement well for the physiologically relevant temperature assay. Furthermore, this probe was applied to map intracellular temperature inside living cells. The fast and accurate temperature response of the probe makes it a promising tool for monitoring the intracellular temperature change, which will promote a better understanding of the temperature relevant biological processes inside living cells.

Graphical abstract: Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots

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Publication details

The article was received on 29 Jan 2017, accepted on 03 Apr 2017 and first published on 04 Apr 2017


Article type: Paper
DOI: 10.1039/C7TB00309A
Citation: J. Mater. Chem. B, 2017,5, 3383-3390
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    Living cell intracellular temperature imaging with biocompatible dye-conjugated carbon dots

    L. Wei, Y. Ma, X. Shi, Y. Wang, X. Su, C. Yu, S. Xiang, L. Xiao and B. Chen, J. Mater. Chem. B, 2017, 5, 3383
    DOI: 10.1039/C7TB00309A

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