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Issue 15, 2017
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A two-photon fluorescent probe for viscosity imaging in vivo

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Abstract

A new two-photon fluorescent probe (MCN) for viscosity imaging was developed based on a 6-substituted quinoline framework. MCN showed an excellent “off–on” fluorescence response (ca. 90-fold enhancement) with viscosity increasing in the glycerol–water viscosity system. MCN showed great sensitivity to viscosity (R2 = 0.98, x = 0.65), which gave rise to cell imaging for micro-viscosity or real-time cell imaging during apoptosis with low cytotoxicity under two-photon excitation (λex = 800 nm). Fluorescence lifetime imaging (FLIM) of living HeLa cells stained with MCN revealed that the intracellular average viscosity value was 73.45 ± 21.55 cP in cytosol. Imaging in living tissue slices indicated that MCN can work in deep tissue (∼130 μM) under two-photon excitation. Moreover, MCN also showed the capacity for in vivo imaging viscosity in zebrafish with obvious fluorescence emission.

Graphical abstract: A two-photon fluorescent probe for viscosity imaging in vivo

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Publication details

The article was received on 12 Jan 2017, accepted on 25 Feb 2017 and first published on 27 Feb 2017


Article type: Paper
DOI: 10.1039/C7TB00136C
Citation: J. Mater. Chem. B, 2017,5, 2743-2749
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    A two-photon fluorescent probe for viscosity imaging in vivo

    P. Ning, P. Dong, Q. Geng, L. Bai, Y. Ding, X. Tian, R. Shao, L. Li and X. Meng, J. Mater. Chem. B, 2017, 5, 2743
    DOI: 10.1039/C7TB00136C

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