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Methylene blue-containing liposomes as new photodynamic anti-bacterial agents

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Abstract

Methylene blue (MB) can be employed as a photo-activatable antimicrobial drug in photodynamic therapy (PDT) due to its ability to release oxygen free radicals upon photo-activation. However, its poor ability to penetrate bacterial cell walls and bacterial biofilms limits its antimicrobial activity. To overcome these limitations, we propose some formulations of MB based on different cationic liposomes. The liposome–MB systems were characterized using dynamic light scattering (DLS), zeta potential analysis and UV-visible spectroscopy. Their ability to penetrate inside the cytoplasm of E. coli, taken as a bacterial model for Gram-negative strains, was investigated through laser scanning confocal microscopy (CLSM) and compared to the penetration of naked MB. Then, MB-loaded liposomes were photo-activated and their antimicrobial activity was tested against E. coli, showing a strong improvement with respect to MB solutions. The liposomal formulations dramatically enhance MB penetration in bacterial biofilms and reduce the inflammatory response due to lipopolysaccharide exposure in mammalian cells. The observed antimicrobial and anti-inflammatory efficacies show a clear correlation with some structural features of the carriers, namely the size and the surface charge density. Overall, these results provide fundamental knowledge that enables the design of novel efficient PDT treatments, which potentially overcome the rising incidence of antibiotic resistance of bacterial strains.

Graphical abstract: Methylene blue-containing liposomes as new photodynamic anti-bacterial agents

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Publication details

The article was received on 27 Dec 2016, accepted on 14 Mar 2017, published on 15 Mar 2017 and first published online on 15 Mar 2017


Article type: Paper
DOI: 10.1039/C6TB03367A
Citation: J. Mater. Chem. B, 2017, Advance Article
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    Methylene blue-containing liposomes as new photodynamic anti-bacterial agents

    G. Boccalini, L. Conti, C. Montis, D. Bani, A. Bencini, D. Berti, C. Giorgi, A. Mengoni and B. Valtancoli, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C6TB03367A

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