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A highly specific and ultrasensitive fluorescent probe for basal lysosomal HOCl detection based on chlorination induced by chlorinium ions (Cl+)

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Abstract

The development of techniques for detecting HOCl at the subcellular level is very important to elucidate its cellular functions. Due to its relatively low concentration, it is still a great challenge to specifically track the basal HOCl in normal cells. In this paper, based on the unique chlorination of HOCl by the initiation of chlorinium ions (Cl+) in an acidic medium, we have developed a simple pH-mediated lysosome-targetable fluorescent probe Lyso-HOCl for the specific detection of HOCl over other bioactive molecules at higher concentration (500 μM). Our results show that Lyso-HOCl possesses a detection limit of 8.0 pM, and can quantitatively detect HOCl at the picomolar level. The ultrasensitive and ultrafast response property of probe Lyso-HOCl offers a good opportunity to monitor the basal HOCl and the fluctuation of endogenous HOCl levels in the lysosomes of macrophages (Raw 264.7 cells), and we thus anticipate that this probe would provide a promising tool for further unraveling the biological functions of HOCl in subcellular lysosomes.

Graphical abstract: A highly specific and ultrasensitive fluorescent probe for basal lysosomal HOCl detection based on chlorination induced by chlorinium ions (Cl+)

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Publication details

The article was received on 24 Dec 2016, accepted on 03 Apr 2017 and first published on 03 Apr 2017


Article type: Paper
DOI: 10.1039/C6TB03345H
Citation: J. Mater. Chem. B, 2017, Advance Article
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    A highly specific and ultrasensitive fluorescent probe for basal lysosomal HOCl detection based on chlorination induced by chlorinium ions (Cl+)

    Y. Wang, L. Wu, C. Liu, B. Guo, B. Zhu, Z. Wang, Q. Duan, Z. Ma and X. Zhang, J. Mater. Chem. B, 2017, Advance Article , DOI: 10.1039/C6TB03345H

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