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A highly specific and ultrasensitive fluorescent probe for basal lysosomal HOCl detection based on the chlorination induced by chlorinium ion (Cl+)

Abstract

The development of techniques for detecting HOCl at the subcellular level is very important to elucidate its cellular functions. Due to relatively low concentration, it is still a great challenge to specifically track the basal HOCl in normal cells. In this paper, based on the unique chlorination of HOCl by the initiation of chlorinium ion (Cl+) in the acidic medium, we have developed a simple pH-mediated lysosome-targetable fluorescent probe Lyso-HOCl for the specific detection of HOCl over other bioactive molecules at higher concentration (500 μM). Our results show that Lyso-HOCl possesses a detection limit of 8.0 pM, and can quantitatively detect HOCl at the picomolar level. The ultrasensitive and ultrafast response property offers probe Lyso-HOCl a pleasant opportunity to monitor the basal HOCl and the fluctuation of endogenous HOCl levels in the lysosomes of macrophages (Raw264.7 cells), and we thus anticipate that this probe would provide a promising tool for further unraveling the biological functions of HOCl in the subcellular lysosomes.

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Publication details

The article was received on 24 Dec 2016, accepted on 03 Apr 2017 and first published on 03 Apr 2017


Article type: Paper
DOI: 10.1039/C6TB03345H
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    A highly specific and ultrasensitive fluorescent probe for basal lysosomal HOCl detection based on the chlorination induced by chlorinium ion (Cl+)

    Y. Wang, L. Wu, C. Liu, B. Guo, B. Zhu, Z. Wang, Q. Duan, Z. Ma and X. Zhang, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C6TB03345H

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