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Issue 14, 2017
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New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani

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Abstract

In order to address the existing limitations of the commercially available fluorescent probe CMAC (7-amino-4-chloromethylcoumarin), a new series of highly fluorescent donor–acceptor 7-hydroxy-coumarin derivatives was prepared and these derivatives were used as vacuole specific fluorescent probes for live cell imaging of unicellular parasitic protozoa L. donovani promastigotes and yeast cells S. pombe and S. cerevisiae. The synthesized 7-hydroxy-coumarins exhibited interesting photophysical properties and have advantages such as excitation in the visible region, good water solubility, photo-stability, good quantum yield and low cytotoxicity.

Graphical abstract: New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani

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Publication details

The article was received on 15 Dec 2016, accepted on 06 Mar 2017 and first published on 06 Mar 2017


Article type: Paper
DOI: 10.1039/C6TB03257E
Citation: J. Mater. Chem. B, 2017,5, 2580-2587
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    New visible light excitable donor–acceptor 7-hydroxy-coumarins as blue fluorescent probes for selective staining of vacuoles in yeasts and L. donovani

    A. Raghuvanshi, A. Kumar Jha, M. Kathuria, B. Priya Awasthi, D. Purohit, K. Mitra and A. Goel, J. Mater. Chem. B, 2017, 5, 2580
    DOI: 10.1039/C6TB03257E

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