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Issue 12, 2017
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Gene delivery to mammalian cells using a graphene nanoribbon platform

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Abstract

We have developed a novel oxidized graphene nanoribbon-based platform (O-GNR) for gene delivery of double-stranded DNA into mammalian cells. O-GNRs, synthesized via longitudinal unzipping of multi-walled carbon nanotubes (MWCNTs), exhibited efficient DNA loading of small dsDNA fragments. Fourier Transform Infrared Spectroscopy identified stretching peaks in the O-P-O and DNA sugar phosphate backbone that were consistent with DNA loading onto O-GNRs. The presence of salts in the loading buffer promoted DNA loading and effective dispersion of O-GNRs. DNA:O-GNR complexes were stable upon treatment with surfactants Tween 20 and Triton-X100. O-GNRs did not impact the viability of mammalian cells. Last, the detection of GFP expression upon transfection of the DNA:O-GNR complex indicated that the cargo DNA is expressed in the nucleus. Taken together, O-GNRs function as a platform for gene delivery to mammalian cells.

Graphical abstract: Gene delivery to mammalian cells using a graphene nanoribbon platform

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Publication details

The article was received on 18 Nov 2016, accepted on 06 Mar 2017 and first published on 06 Mar 2017


Article type: Paper
DOI: 10.1039/C6TB03010F
Citation: J. Mater. Chem. B, 2017,5, 2347-2354
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    Gene delivery to mammalian cells using a graphene nanoribbon platform

    H. C. Foreman, G. Lalwani, J. Kalra, L. T. Krug and B. Sitharaman, J. Mater. Chem. B, 2017, 5, 2347
    DOI: 10.1039/C6TB03010F

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