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A novel biomimetic scaffolds with hUCMSCs for lumbar fusion

Abstract

Discectomy and lumber fusion are common clinical approaches to treating intervertebral disc (IVD) degeneration by the aid of autologous bone and/or biomaterials. Biomaterials are considered suitable if they possess biodegradability and ability to guide tissue regeneration. These features are very necessary to move the whole IVD when it degenerates as treatment for lumbar fusion because the use of autogenous bone can produce secondary damage. In this work, a novel biomimetic porous chitosan/ poly (L-lactic acid) scaffolds with human umbilical cord mesenchymal stem cells (hUCMSCs) are applied in lumbar fusion for the first time the experiment processes are carried out in accordance with the whole flow chart shown as Fig. 1. Hierarchically porous chitosan scaffolds were prepared using porogen and freeze drying, and then poly (L-lactic acid) networks were distributed throughout the scaffold interior to enhance mechanical strength. We conducted in vivo experiments in animals, in which the scaffolds were first incubated in hUCMSC solution for 8 days and then implanted into half-destroyed IVDs in New Zealand rabbits. Results showed that scaffolds with hUCMSCs had greater ability to guide disc regeneration than blank control and autologous bone as determined using X-ray, computed tomography, and histologic analyses. Findings regarding the osteogenic sources for the stem cell repair of bone defects confirmed the role of hUCMSC stimulation in bone-tissue healing. This hMUMSC-based approach together with the strategy proposed for incorporating osteoblastic cells into scaffolds that promote endogenous or synthetic repair mechanisms may then be used to develop strategies for the stem-cell-based healing of other acute injuries and chronic diseases.

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Publication details

The article was received on 10 Oct 2016, accepted on 26 May 2017 and first published on 26 May 2017


Article type: Paper
DOI: 10.1039/C6TB02640K
Citation: J. Mater. Chem. B, 2017, Accepted Manuscript
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    A novel biomimetic scaffolds with hUCMSCs for lumbar fusion

    N. Li, Z. Li , R. Li, J. Tian, G. Sun, L. Li, D. Wu, S. Ding and C. Zhou, J. Mater. Chem. B, 2017, Accepted Manuscript , DOI: 10.1039/C6TB02640K

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