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Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(α-amino acids)

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Abstract

In this study, the potential of highly porous hydrogels based on biodegradable synthetic poly(α-amino acids) to support proliferation and chondrogenesis of human dental pulp stem cells (hDPSCs) was investigated. Covalently crosslinked gels with permanent pores were formed under cryogenic conditions by free-radical copolymerization of poly[N5-(2-hydroxyethyl)-L-glutamine-stat-N5-(2-methacryloyl-oxy-ethyl)-L-glutamine] (PHEG-MA) with 2-hydroxyethyl methacrylate (HEMA) and N-propargyl methacrylamide (PrMAAm) as minor co-monomers. PrMAAm provided alkyne groups for modifying the gels with cell-supporting moieties (RGDS peptides) by the azide–alkyne “click”-reaction. Two types of gels with different compressive moduli were prepared. Each type was modified with two different concentrations of RGDS peptide. X-ray computed nanotomography (nanoCT) was used to visualize and analyze the 3D-structure of the cryogels. It was shown that modifying the PHEG-MA cryogels within the range of RGDS concentrations examined here had a positive effect on the proliferation of hDPSCs. Immunofluorescence staining for collagen type 2 and aggrecan proved that there was differentiation of hDPSCs into chondrocytes.

Graphical abstract: Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(α-amino acids)

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Publication details

The article was received on 20 Oct 2017, accepted on 30 Nov 2017 and first published on 30 Nov 2017


Article type: Paper
DOI: 10.1039/C7SM02074K
Citation: Soft Matter, 2018, Advance Article
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    Chondrogenic potential of macroporous biodegradable cryogels based on synthetic poly(α-amino acids)

    T. Sedlačík, O. K. Acar, H. Studenovská, I. Kotelnikov, J. Kučka, Z. Konečná, T. Zikmund, J. Kaiser, G. T. Kose and F. Rypáček, Soft Matter, 2018, Advance Article , DOI: 10.1039/C7SM02074K

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