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Templated co-assembly into nanorods of polyanions and artificial virus capsid proteins

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Abstract

Recombinant triblock polypeptides C–Sn–B, where C is a 400 amino acid long hydrophilic random coil block, Sn is a multimer of the silk-like octapeptide S = (GAGAGAGQ), and B = K12 is an oligolysine, have previously been shown to encapsulate double stranded DNA into rod-shaped, virus-like particles. In order to gain insight of the co-assembly process, and in order to be able to use these proteins for templating other types of nanorods, we here explore their co-assembly with a range of polyanionic templates: poly(acrylic acids) (PAA) of a wide range of lengths, poly(styrene sulphonate) (PSS) and the stiff anionic polysaccharide xanthan. The formation of the complexes was characterized using Dynamic Light Scattering (DLS), cryogenic Transmission Electronic Microscopy (Cryo-TEM) and Atomic Force Microscopy (AFM). Except at very high molar masses, we find that flexible anionic PAA and PSS lead to co-assembly of proteins with single polyanion chains into nanorods, with a packing factor as expected on the basis of charge stochiometry. Only for very long PAA templates (8 × 105 Da) we find evidence for heterogeneous complexes with thin and thick sections. For the very stiff xanthan chains, we find that its stiffness precludes co-assembly with the artificial viral capsid proteins into condensed and regular nanorods. Given the simple and robust formation of rod-like structures with a range of polyanionic templates, we anticipate that the artificial virus proteins will be useful for preparing high-aspect ratio nanoparticles and scaffolds of precise size and find applications in nanotechnology and materials science for which currently natural rod-like viruses are being explored.

Graphical abstract: Templated co-assembly into nanorods of polyanions and artificial virus capsid proteins

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Publication details

The article was received on 10 Oct 2017, accepted on 28 Nov 2017 and first published on 29 Nov 2017


Article type: Paper
DOI: 10.1039/C7SM02012K
Citation: Soft Matter, 2018, Advance Article
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    Templated co-assembly into nanorods of polyanions and artificial virus capsid proteins

    A. Hernandez-Garcia, M. A. Cohen Stuart and R. de Vries, Soft Matter, 2018, Advance Article , DOI: 10.1039/C7SM02012K

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