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Issue 48, 2017
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Centrifugation-induced fibrous orientation in fish-sourced collagen matrices

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Abstract

Orientation of fibrous collagen structures plays an important role not only in the native function of various biological tissues but also in the development of next-generation tissue engineering scaffolds. However, the controlled assembly of collagen in vitro into an anisotropic structure, avoiding complex technical procedures and specialized apparatus, remains a challenge. Here, an oriented collagen matrix was fabricated at the macroscale by simple centrifugation, and the aligned topographical features of the resulting collagen matrix were revealed by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and small angle X-ray scattering. The aligned matrix exhibited a higher ultimate tensile strength and strain than a random matrix. Centrifugation had an impact on the diameter and density of the collagen fibrils, while it had no effect on their native D-periodicity and thermal stability. Additionally, structural anisotropy of the collagen matrix facilitated the proliferation and migration of NIH/3T3 fibroblasts, compared with the random one. This simple and cost-effective method could lead to mass production of aligned collagen matrices and future possibilities for different applications in tissue engineering.

Graphical abstract: Centrifugation-induced fibrous orientation in fish-sourced collagen matrices

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Publication details

The article was received on 17 Sep 2017, accepted on 22 Nov 2017 and first published on 22 Nov 2017


Article type: Paper
DOI: 10.1039/C7SM01871A
Citation: Soft Matter, 2017,13, 9220-9228
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    Centrifugation-induced fibrous orientation in fish-sourced collagen matrices

    J. Zhang, Y. Sun, Y. Zhao, B. Wei, C. Xu, L. He, C. L. P. Oliveira and H. Wang, Soft Matter, 2017, 13, 9220
    DOI: 10.1039/C7SM01871A

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