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Self-assembled selenium nanoparticles and their application in the rapid diagnostic detection of small cell lung cancer biomarkers

Abstract

By coupling molecular imprinting, chitosan biosorption and TiO2 photocatalysis, selenium nanoparticles (Se NPs) were self-assembled in a controlled manner on the molecular imprinting sites of zeolite-chitosan-TiO2 microspheres. Se NPs with different size ranges and areal densities were individually synthesized by controlling the rapid adsorption of molecular-imprinted nanocomposites and photocatalytic reaction of TiO2 nanoparticles. In order to improve the sensitivity and specificity of rapid diagnostic detection, Se NPs were self-assembled again into high-order and spherical stable structures with an average size of 80 nm by well-defined monomer units, after separation form zeolite-chitosan-TiO2 microspheres with a stabilizer of 0.3% (v/v) bovine serum albumin. Due to the biological activity, spherical-shaped Se NPs were used for dot-blot immunoassays with multiple native antigens for rapid serodiagnosis of human lung cancer. The sensitivity of the dot immunoassays for detecting progastrin-releasing peptide (ProGRP) was 75 pg/mL. The detection time of colloidal Se dot immunoassays for ProGRP was only 5 min. No positive results were observed with other commonly potential interfering substances, including carcinoembryonic antigen, α-fetoprotein antigen and BSA. The research presents a simple and green method for the reuse of SeO32- and controlled synthesis of Se NPs for biological and medical applications by bioaffinity adsorption and photoreduction.

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Publication details

The article was received on 22 Aug 2017, accepted on 06 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7SM01687E
Citation: Soft Matter, 2017, Accepted Manuscript
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    Self-assembled selenium nanoparticles and their application in the rapid diagnostic detection of small cell lung cancer biomarkers

    Y. Zhao, Q. Sun, X. Zhang, J. Baeyens and H. Su, Soft Matter, 2017, Accepted Manuscript , DOI: 10.1039/C7SM01687E

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