Jump to main content
Jump to site search

Issue 31, 2017
Previous Article Next Article

Impact of a mechanical shear stress on intracellular trafficking

Author affiliations

Abstract

Intracellular trafficking mainly takes place along the microtubules, and its efficiency depends on the local architecture and organization of the cytoskeletal network. In this work, the cytoplasm of stem cells is subjected to mechanical vortexing at a frequency of up to 1 Hz, by using magnetic chains of endosomes embedded in the cell body, in order to locally perturb the network structure. The consequences are evaluated on the directionality and processivity of the spontaneous motion of endosomes. When the same chains are used both to shear the cell medium and to probe the intracellular traffic, a substantial decrease in transport efficiency is detected after applying the mechanical shear. Interestingly, when using different objects to apply the shear and to probe the spontaneous motion, no alteration of the transport efficiency can be detected. We conclude that shaking the vesicles mainly causes their unbinding from the cytoskeletal tracks, but has little influence on the integrity of the network itself. This is corroborated by active microrheology measurements, performed with chains actuated by a magnetic field, and showing that the mechanical compliance of the cytoplasm is similar before and after slow vortexing.

Graphical abstract: Impact of a mechanical shear stress on intracellular trafficking

Back to tab navigation

Supplementary files

Publication details

The article was received on 12 Apr 2017, accepted on 16 Jun 2017 and first published on 23 Jun 2017


Article type: Paper
DOI: 10.1039/C7SM00732A
Citation: Soft Matter, 2017,13, 5298-5306
  •   Request permissions

    Impact of a mechanical shear stress on intracellular trafficking

    K. Aubertin, J. Tailleur, C. Wilhelm and F. Gallet, Soft Matter, 2017, 13, 5298
    DOI: 10.1039/C7SM00732A

Search articles by author

Spotlight

Advertisements