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Issue 34, 2017
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Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture

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Abstract

We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24–4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy. The pattern was successfully transferred down to 0.37 μm periodicity (width in ELP: 250 ± 50 nm, height: 70 ± 40 nm). The limit was set by inherent protein assemblies (diameter: 124–180 nm) that formed due to lower critical solution temperature behavior of the ELP during molding. The width/height of the ELP ridges depended on the degree of hydration; from complete dehydration to full hydration, ELP ridge width ranged from 79 ± 9% to 150 ± 40% of the mold width. The surface of the ridged ELP featured densely packed protein aggregates that were larger in size than those observed in bulk/flat ELP. Adipose-derived stem cells (ADSCs) oriented along hydrated aligned patterns with periodicities ≥0.60 μm (height ≥170 ± 100 nm), while random orientation was observed for smaller distances/amplitudes, as well as flat and unaligned wavy ELP surfaces. Hence, micro-molding of ELP is a promising approach to create tissue-mimicking, hierarchical architectures composed of tunable micron-sized structures with nano-sized protein aggregates, which opens the way for orthogonal screening of cell responses to topography and cell-adhesion ligands at relevant elastic moduli.

Graphical abstract: Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture

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Publication details

The article was received on 09 Mar 2017, accepted on 14 Jul 2017 and first published on 17 Jul 2017


Article type: Paper
DOI: 10.1039/C7SM00487G
Citation: Soft Matter, 2017,13, 5665-5675
  • Open access: Creative Commons BY-NC license
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    Micro- and nano-patterned elastin-like polypeptide hydrogels for stem cell culture

    A. Paul, M. Stührenberg, S. Chen, D. Rhee, W.-K. Lee, T. W. Odom, S. C. Heilshorn and A. Enejder, Soft Matter, 2017, 13, 5665
    DOI: 10.1039/C7SM00487G

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