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Issue 17, 2017
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Glycolipid-based nanostructures with thermal-phase transition behavior functioning as solubilizers and refolding accelerators for protein aggregates

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Abstract

The self-assembly of synthetic glycolipids produced nanostructures such as vesicles and nanotubes consisting of bilayer membranes, which underwent a gel-to-liquid crystalline thermal phase transition. Vesicles formed at temperatures above the thermal phase transition temperatures (Tg–l) could solubilize aggregates of denatured proteins by trapping them in the fluid bilayer membranes. Cooling to temperatures below Tg–l caused a morphological transformation into nanotubes that accompanied the thermal phase transition from the fluid to the solid state. This phenomenon allowed the trapped proteins to be quickly released into the bulk solution and simultaneously facilitated the refolding of the proteins. The refolding efficiency strongly depended on the electrostatic attraction between the bilayer membranes of the nanostructures and the proteins. Because of the long shape (>400 nm) of the nanotubes, simple membrane filtration through a pore size of 200 nm led to complete separation and recovery of the refolded proteins (3–9 nm sizes).

Graphical abstract: Glycolipid-based nanostructures with thermal-phase transition behavior functioning as solubilizers and refolding accelerators for protein aggregates

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Publication details

The article was received on 14 Feb 2017, accepted on 21 Mar 2017 and first published on 23 Mar 2017


Article type: Paper
DOI: 10.1039/C7SM00310B
Citation: Soft Matter, 2017,13, 3084-3090
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    Glycolipid-based nanostructures with thermal-phase transition behavior functioning as solubilizers and refolding accelerators for protein aggregates

    N. Kameta, T. Matsuzawa, K. Yaoi, J. Fukuda and M. Masuda, Soft Matter, 2017, 13, 3084
    DOI: 10.1039/C7SM00310B

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