Jump to main content
Jump to site search

Issue 23, 2017
Previous Article Next Article

Optical sensing of mechanical pressure based on diffusion measurement in polyacrylamide cell-like barometers

Author affiliations

Abstract

Diffusion and transport of small molecules within hydrogel networks are of high interest for biomedical and pharmaceutical research. Herein, using fluorescence correlation spectroscopy (FCS), we experimentally showed that the diffusion time in the hydrogel was directly related to the mechanical state (compression or swelling) and thus to the volume fraction of the gel. Following this observation, we developed cell-like barometers in the form of PAA microbeads, which when incorporated between cells and combined with a diffusion-based optical readout could serve as the first biosensors to measure the local pressure inside the growing biological tissues. To illustrate the potential of the present method, we used multicellular spheroids (MCS) as a tissue model, and it was observed that the growth-associated tissue stress was lower than 1 kPa, but significantly increased when an external compressive stress was applied.

Graphical abstract: Optical sensing of mechanical pressure based on diffusion measurement in polyacrylamide cell-like barometers

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Dec 2016, accepted on 01 May 2017 and first published on 05 Jun 2017


Article type: Communication
DOI: 10.1039/C6SM02887J
Citation: Soft Matter, 2017,13, 4210-4213
  •   Request permissions

    Optical sensing of mechanical pressure based on diffusion measurement in polyacrylamide cell-like barometers

    F. Ingremeau, M. E. Dolega, J. Gallagher, I. Wang, G. Cappello and A. Delon, Soft Matter, 2017, 13, 4210
    DOI: 10.1039/C6SM02887J

Search articles by author

Spotlight

Advertisements