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Sustainable production of medium chain fatty acids (MCFA) with an enriched mixed bacterial culture: microbial characterization using molecular methods

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Abstract

Chain elongation is the process by which bacteria convert ethanol and short chain fatty acids (SCFA) into medium chain fatty acids (MCFA). In the present study, a bacterial mixed culture was enriched and used together with synthetic waste (SW) to produce commercially valuable SCFA and MCFA for 91 days by anaerobic fermentation. For the first time, the effects of substrate, electron donor, and methane inhibition on MCFA production were evaluated. The produced SCFA, MCFA and biogas were analyzed using chromatography techniques. Butyrate (C4) and caproate (C6) were the dominant products in SCFA and MCFA, respectively. Bacteria in conditions of methane inhibition produced the highest concentration of butyrate (8.2 g l−1), caproate (8.6 g l−1), heptanoate (0.7 g l−1), and caprylate (0.23 g l−1). The dominance of Clostridia, Rumen bacterium, and the Actinomyces group in the enriched culture were identified by denaturing gradient gel electrophoresis (DGGE) analysis. Studies are being conducted under pipeline with enriched cultures to produce SCFA and MCFA using the real waste streams.

Graphical abstract: Sustainable production of medium chain fatty acids (MCFA) with an enriched mixed bacterial culture: microbial characterization using molecular methods

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Publication details

The article was received on 24 Sep 2017, accepted on 03 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7SE00467B
Citation: Sustainable Energy Fuels, 2018, Advance Article
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    Sustainable production of medium chain fatty acids (MCFA) with an enriched mixed bacterial culture: microbial characterization using molecular methods

    M. V. Reddy, S. V. Mohan and Y. Chang, Sustainable Energy Fuels, 2018, Advance Article , DOI: 10.1039/C7SE00467B

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