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Sustainable production of medium chain fatty acids (MCFA) with enriched mixed bacterial culture: microbial characterization using molecular methods

Abstract

Chain elongation is the process where bacteria convert ethanol and short chain fatty acids (SCFA) into medium chain fatty acids (MCFA). In the present study, bacterial mixed culture was enriched, and using this enriched culture and synthetic waste (SW) commercially valuable SCFA and MCFA were produced for 91 days under anaerobic fermentation. For the first time, effect of substrate, electron donor, and methane inhibition on MCFA production was evaluated. Produced SCFA, MCFA and biogas were analysed using chromatography techniques. Butyrate (C4), and caproate (C6) were the dominant products in SCFA and MCFA respectively. Bacteria with methane inhibition condition showed highest concentration of butyrate (8.2 g/l), caproate (8.6 g/l), heptanoate (0.7 g/l), and caprylate (0.23 g/l). Dominance of Clostridia, Rumen bacterium, and Actinomyces group in the enriched culture are identified by denaturing gradient gel electrophoresis (DGGE) analysis. Studies were under pipeline with enriched culture to produce SCFA and MCFA using the real waste streams.

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Publication details

The article was received on 24 Sep 2017, accepted on 03 Nov 2017 and first published on 06 Nov 2017


Article type: Paper
DOI: 10.1039/C7SE00467B
Citation: Sustainable Energy Fuels, 2017, Accepted Manuscript
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    Sustainable production of medium chain fatty acids (MCFA) with enriched mixed bacterial culture: microbial characterization using molecular methods

    M. Venkateswar Reddy, S. Venkata Mohan and Y. Chang, Sustainable Energy Fuels, 2017, Accepted Manuscript , DOI: 10.1039/C7SE00467B

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