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Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification

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Abstract

Herein, we design a smart autonomous ATP self-powered strand-displacement cascade amplification (SDCA) system for highly sensitive multiple intracellular miRNA detection. Rationally engineered Y-motif DNA structures are functionalized on mesoporous silica-coated copper sulfide nanoparticles loaded with numerous ATPs (CuS@mSiO2-Y/ATP) through pH stimulus-responsive disulfide bonds. The SDCA system is implemented by endogenous specific miRNA as a trigger and ATP as fuel released from the nanocarrier at acidic pH and photothermal stimuli-responsive CuS. The ATP self-powered SDCA process presents higher sensitivity compared to that without amplification for intracellular miRNA imaging. Two-color simultaneous and sensitive imaging of multiple cancer-related miRNAs in living cells is also confirmed. This enables facile and accurate differentiation between normal cells and different types of cancer cell using intracellular miRNA imaging, which improves the veracity and timeliness for early cancer diagnosis.

Graphical abstract: Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification

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Publication details

The article was received on 01 Nov 2017, accepted on 30 Nov 2017 and first published on 01 Dec 2017


Article type: Edge Article
DOI: 10.1039/C7SC04725H
Citation: Chem. Sci., 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Imaging multiple microRNAs in living cells using ATP self-powered strand-displacement cascade amplification

    X. Meng, W. Dai, K. Zhang, H. Dong and X. Zhang, Chem. Sci., 2018, Advance Article , DOI: 10.1039/C7SC04725H

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