Jump to main content
Jump to site search


Supramolecular assembly affording a ratiometric two-photon fluorescent nanoprobe for quantitative detection and bioimaging

Author affiliations

Abstract

Fluorescence quantitative analyses for vital biomolecules are in great demand in biomedical science owing to their unique detection advantages with rapid, sensitive, non-damaging and specific identification. However, available fluorescence strategies for quantitative detection are usually hard to design and achieve. Inspired by supramolecular chemistry, a two-photon-excited fluorescent supramolecular nanoplatform (TPSNP) was designed for quantitative analysis with three parts: host molecules (β-CD polymers), a guest fluorophore of sensing probes (Np–Ad) and a guest internal reference (NpRh–Ad). In this strategy, the TPSNP possesses the merits of (i) improved water-solubility and biocompatibility; (ii) increased tissue penetration depth for bioimaging by two-photon excitation; (iii) quantitative and tunable assembly of functional guest molecules to obtain optimized detection conditions; (iv) a common approach to avoid the limitation of complicated design by adjustment of sensing probes; and (v) accurate quantitative analysis by virtue of reference molecules. As a proof-of-concept, we utilized the two-photon fluorescent probe NHS–Ad-based TPSNP-1 to realize accurate quantitative analysis of hydrogen sulfide (H2S), with high sensitivity and good selectivity in live cells, deep tissues and ex vivo-dissected organs, suggesting that the TPSNP is an ideal quantitative indicator for clinical samples. What’s more, TPSNP will pave the way for designing and preparing advanced supramolecular sensors for biosensing and biomedicine.

Graphical abstract: Supramolecular assembly affording a ratiometric two-photon fluorescent nanoprobe for quantitative detection and bioimaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Sep 2017, accepted on 06 Oct 2017 and first published on 09 Oct 2017


Article type: Edge Article
DOI: 10.1039/C7SC03977H
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Supramolecular assembly affording a ratiometric two-photon fluorescent nanoprobe for quantitative detection and bioimaging

    P. Wang, C. Zhang, H. Liu, M. Xiong, S. Yin, Y. Yang, X. Hu, X. Yin, X. Zhang and W. Tan, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC03977H

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements