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Simultaneously quantify multiple endogenous biothiols in single living cells by plasmonic Raman probes

Abstract

Intracellular biothiols mediate many important physiological and pathological progresses. Due to the low content and their competing thiol-reactivity, it is still an unmet challenge to quantify them within complicated intracellular environment. Herein, we demonstrated a strategy to discriminate three biothiols, i.e. cysteine (Cys), homo-cysteine (Hcy) and glutathione (GSH), and quantify their concentrations within single living cells, by using one platform of Raman probe. By monitoring the reactive kinetics of biothiols with Raman probes, and discriminating their products with a quantitative principal component analysis (qPCA) method, these three biothiols could be simultaneously quantified in both cell lysis and single living cells. The concentrations of Cys, Hcy and GSH in single Hela cells were 158 ± 19 μM, 546 ± 67 μM and 5.07 ± 0.62 mM, respectively, which gives the precise concentrations of these three biothiols at single cell level for the first time. This method provides a general strategy of discriminating each component from a mixed system, and has potentials for quantifying any biomolecules within in vitro or in vivo biological environment.

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Publication details

The article was received on 24 Jul 2017, accepted on 28 Aug 2017 and first published on 29 Aug 2017


Article type: Edge Article
DOI: 10.1039/C7SC03218H
Citation: Chem. Sci., 2017, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Simultaneously quantify multiple endogenous biothiols in single living cells by plasmonic Raman probes

    S. Li, Q. Guan, M. Zheng, Y. Wang, D. Ye, B. Kang, J. Xu and H. Chen, Chem. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7SC03218H

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