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A platform for high-throughput screening of DNA-encoded catalyst libraries in organic solvents

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Abstract

We have developed a novel high-throughput screening platform for the discovery of small-molecules catalysts for bond-forming reactions. The method employs an in vitro selection for bond-formation using amphiphilic DNA-encoded small molecules charged with reaction substrate, which enables selections to be conducted in a variety of organic or aqueous solvents. Using the amine-catalysed aldol reaction as a catalytic model and high-throughput DNA sequencing as a selection read-out, we demonstrate the 1200-fold enrichment of a known aldol catalyst from a library of 16.7-million uncompetitive library members.

Graphical abstract: A platform for high-throughput screening of DNA-encoded catalyst libraries in organic solvents

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Publication details

The article was received on 22 Jun 2017, accepted on 20 Aug 2017 and first published on 21 Aug 2017


Article type: Edge Article
DOI: 10.1039/C7SC02779F
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY license
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    A platform for high-throughput screening of DNA-encoded catalyst libraries in organic solvents

    K. D. Hook, J. T. Chambers and R. Hili, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC02779F

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