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Issue 10, 2017
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Immuno-targeting of Staphylococcus aureus via surface remodeling complexes

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Abstract

Agents with novel mechanisms of action are needed to complement traditional antibiotics. Towards these goals, we have exploited the surface-homing properties of vancomycin to tag the surface of Gram-positive pathogens with immune cell attractants in two unique modes. First, vancomycin was conjugated to the small molecule hapten 2,4-dinitrophenol (DNP) to promote bacterial opsonization. Second, we built on these results by improving the tagging specificity and mechanism of incorporation by coupling it to a sortase A substrate peptide. We demonstrated, for the first time, that the surface of Staphylococcus aureus (S. aureus) can be metabolically labeled in live Caenorhabditis elegans hosts. These constructs represent a class of promising narrow-spectrum agents that target S. aureus for opsonization and establish a new surface labeling modality in live host organisms, which should be a powerful tool in dissecting features of host–pathogen interactions.

Graphical abstract: Immuno-targeting of Staphylococcus aureus via surface remodeling complexes

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Publication details

The article was received on 19 Jun 2017, accepted on 20 Aug 2017 and first published on 23 Aug 2017


Article type: Edge Article
DOI: 10.1039/C7SC02721D
Citation: Chem. Sci., 2017,8, 6804-6809
  • Open access: Creative Commons BY-NC license
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    Immuno-targeting of Staphylococcus aureus via surface remodeling complexes

    M. J. Sabulski, S. E. Pidgeon and Marcos M. Pires, Chem. Sci., 2017, 8, 6804
    DOI: 10.1039/C7SC02721D

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