Jump to main content
Jump to site search


Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption

Author affiliations

Abstract

Genetically encoded covalent peptide tagging technology, such as the SpyTag–SpyCatcher reaction, has emerged as a unique way to do chemistry with proteins. Herein, we report the reactivity engineering of SpyTag–SpyCatcher mutant pairs and show that distinct reactivity can be encrypted for the same reaction based on protein sequences of high similarity. Valuable features, including high selectivity, inverse temperature dependence and (nearly) orthogonal reactivity, could be achieved based on as few as three mutations. This demonstrates the robustness of the SpyTag–SpyCatcher reaction and the plasticity of its sequence specificity, pointing to a family of engineered protein chemistry tools.

Graphical abstract: Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Jun 2017, accepted on 18 Jul 2017 and first published on 19 Jul 2017


Article type: Edge Article
DOI: 10.1039/C7SC02686B
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY license
  •   Request permissions

    Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption

    Y. Liu, D. Liu, W. Yang, X. Wu, L. Lai and W. Zhang, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC02686B

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements