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Issue 8, 2017
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Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

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Abstract

Stimulated Raman scattering (SRS) microscopy in tandem with bioorthogonal Raman labelling strategies is set to revolutionise the direct visualisation of intracellular drug uptake. Rational evaluation of a series of Raman-active labels has allowed the identification of highly active labels which have minimal perturbation on the biological efficacy of the parent drug. Drug uptake has been correlated with markers of cellular composition and cell cycle status, and mapped across intracellular structures using dual-colour and multi-modal imaging. The minimal phototoxicity and low photobleaching associated with SRS microscopy has enabled real-time imaging in live cells. These studies demonstrate the potential for SRS microscopy in the drug development process.

Graphical abstract: Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

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Publication details

The article was received on 25 Apr 2017, accepted on 15 May 2017 and first published on 24 May 2017


Article type: Edge Article
DOI: 10.1039/C7SC01837A
Citation: Chem. Sci., 2017,8, 5606-5615
  • Open access: Creative Commons BY license
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    Imaging drug uptake by bioorthogonal stimulated Raman scattering microscopy

    W. J. Tipping, M. Lee, A. Serrels, V. G. Brunton and A. N. Hulme, Chem. Sci., 2017, 8, 5606
    DOI: 10.1039/C7SC01837A

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