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Amyloid β-peptides 1–40 and 1–42 form oligomers with mixed β-sheets

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Abstract

Two main amyloid-β peptides of different length (Aβ40 and Aβ42) are involved in Alzheimer's disease. Their relative abundance is decisive for the severity of the disease and mixed oligomers may contribute to the toxic species. However, little is know about the extent of mixing. To study whether Aβ40 and Aβ42 co-aggregate, we used Fourier transform infrared spectroscopy in combination with 13C-labeling and spectrum calculation and focused on the amide I vibration, which is sensitive to backbone structure. Mixtures of monomeric labeled Aβ40 and unlabeled Aβ42 (and vice versa) were co-incubated for ∼20 min and their infrared spectrum recorded. The position of the main 13C-amide I′ band shifted to higher wavenumbers with increasing admixture of 12C-peptide due to the presence of 12C-amides in the vicinity of 13C-amides. The results indicate that Aβ40 and Aβ42 form mixed oligomers with a largely random distribution of Aβ40 and Aβ42 strands in their β-sheets. The structures of the mixed oligomers are intermediate between those of the pure oligomers. There is no indication that one of the peptides forces the backbone structure of its oligomers on the other peptide when they are mixed as monomers. We also demonstrate that isotope-edited infrared spectroscopy can distinguish aggregation modulators that integrate into the backbone structure of their interaction partner from those that do not. As an example for the latter case, the pro-inflammatory calcium binding protein S100A9 is shown not to incorporate into the β-sheets of Aβ42.

Graphical abstract: Amyloid β-peptides 1–40 and 1–42 form oligomers with mixed β-sheets

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Publication details

The article was received on 19 Apr 2017, accepted on 12 Oct 2017 and first published on 12 Oct 2017


Article type: Edge Article
DOI: 10.1039/C7SC01743J
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY license
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    Amyloid β-peptides 1–40 and 1–42 form oligomers with mixed β-sheets

    M. Baldassarre, C. M. Baronio, L. A. Morozova-Roche and A. Barth, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC01743J

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