Jump to main content
Jump to site search


Thiol–ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles

Author affiliations

Abstract

Bioconjugation based on crosslinking primary amines to carboxylic acid groups has found broad applications in protein modification, drug development, and nanomaterial functionalization. However, proteins, which are made up of amino acids, typically give nonselective bioconjugation when using primary amine-based crosslinking. In order to control protein orientation and activity after conjugation, selective bioconjugation is desirable. We herein report an efficient and cysteine-selective thiol–ene click reaction-based bioconjugation strategy using colloidal nanoparticles. The resulting thiol–ene based aptamer and enzyme nanoconjugates demonstrated excellent target binding ability and enzymatic activity, respectively. Thus, thiol–ene click chemistry can provide a stable and robust crosslinker in a biocompatible manner for bioconjugation of any thiol-containing biomolecule with nanomaterials. This will open more opportunities for applications of thiol–ene reactions and functional colloidal nanoparticles in chemical biology.

Graphical abstract: Thiol–ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 31 Mar 2017, accepted on 15 Jun 2017 and first published on 22 Jun 2017


Article type: Edge Article
DOI: 10.1039/C7SC01447C
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY license
  •   Request permissions

    Thiol–ene click chemistry: a biocompatible way for orthogonal bioconjugation of colloidal nanoparticles

    Y. Liu, W. Hou, H. Sun, C. Cui, L. Zhang, Y. Jiang, Y. Wu, Y. Wang, J. Li, B. S. Sumerlin, Q. Liu and W. Tan, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC01447C

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements