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Issue 8, 2017
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Dynamic multicolor protein labeling in living cells

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Abstract

Yellow Fluorescence-Activating and absorption-Shifting Tag (Y-FAST, hereafter called FAST) is a 14 kDa protein tag giving a bright green-yellow fluorescent complex upon interaction with the fluorogenic dye 4-hydroxy-3-methylbenzylidene rhodanine (HMBR). Here, we report a collection of fluorogens enabling tuning of the fluorescence color of FAST from green-yellow to orange and red. Beyond allowing the multicolor imaging of FAST-tagged proteins in live cells, these fluorogens enable dynamic color switching because of FAST’s reversible labeling. This unprecedented behavior allows for selective detection of FAST-tagged proteins in cells expressing both green and red fluorescent species through two-color cross-correlation, opening up exciting prospects to overcome spectral crowding and push the frontiers of multiplexed imaging.

Graphical abstract: Dynamic multicolor protein labeling in living cells

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Publication details

The article was received on 27 Mar 2017, accepted on 20 May 2017 and first published on 30 May 2017


Article type: Edge Article
DOI: 10.1039/C7SC01364G
Citation: Chem. Sci., 2017,8, 5598-5605
  • Open access: Creative Commons BY license
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    Dynamic multicolor protein labeling in living cells

    C. Li, M. Plamont, H. L. Sladitschek, V. Rodrigues, I. Aujard, P. Neveu, T. Le Saux, L. Jullien and A. Gautier, Chem. Sci., 2017, 8, 5598
    DOI: 10.1039/C7SC01364G

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