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Issue 10, 2017
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A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

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Abstract

An antibody–AIEgen conjugate is designed and developed as a “turn-on” fluorescent probe for wash-free specific cancer cell imaging. The cetuximab-conjugated AIEgen shows red fluorescence only when it is internalized and accumulated in cancer cells with overexpressed epidermal growth factor receptor through endocytosis. The probe first lights up the lysosomes. After hydrolysis, its residue is accumulated in mitochondria, making them highly emissive with a long cell retention time. Compared with conventional “always-on” fluorescent probes, the antibody–AIEgen conjugate exhibits a very good image contrast during wash-free cancer cell imaging and less interference from normal cells. To the best of our knowledge, this is the first time “turn-on” antibody–AIEgen conjugates have been reported. This new strategy can be further extended to many proteins and water-soluble AIEgens, and many of their potential applications such as real-time tracking of cell dynamics and cancer theranostics will be explored. The present work is expected to inspire more marvellous research in the fields of AIE and cancer imaging.

Graphical abstract: A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

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Publication details

The article was received on 08 Mar 2017, accepted on 07 Aug 2017 and first published on 17 Aug 2017


Article type: Edge Article
DOI: 10.1039/C7SC01054K
Citation: Chem. Sci., 2017,8, 7014-7024
  • Open access: Creative Commons BY license
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    A red-emissive antibody–AIEgen conjugate for turn-on and wash-free imaging of specific cancer cells

    X. Shi, C. Y. Y. Yu, H. Su, R. T. K. Kwok, M. Jiang, Z. He, J. W. Y. Lam and B. Z. Tang, Chem. Sci., 2017, 8, 7014
    DOI: 10.1039/C7SC01054K

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