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Enrichment and fluorogenic labelling of 5-formyluracil in DNA

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Abstract

Recently, the detection of natural thymine modified 5-formyluracil has attracted widespread attention. Herein, we introduce a new insight into designing reagents for both the selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil, under physiological conditions, which can then be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than the fluorescence did itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.

Graphical abstract: Enrichment and fluorogenic labelling of 5-formyluracil in DNA

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Publication details

The article was received on 10 Feb 2017, accepted on 02 Apr 2017 and first published on 05 Apr 2017


Article type: Edge Article
DOI: 10.1039/C7SC00637C
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY license
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    Enrichment and fluorogenic labelling of 5-formyluracil in DNA

    C. Liu, Y. Wang, X. Zhang, F. Wu, W. Yang, G. Zou, Q. Yao, J. Wang, Y. Chen, S. Wang and X. Zhou, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC00637C

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