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Both enrichment and fluorogenic labelling of 5-formyluracil in DNA

Abstract

Recently, the detection of the natural thymine modification 5-formyluracil has attracted widespread attention. Herein, we put forward a new insight into designing reagents for both selective biotin enrichment and fluorogenic labelling of 5-formyluracil in DNA. Biotinylated o-phenylenediamine directly tethered to naphthalimide can switch on 5-formyluracil under physical conditions that can be used in cell imaging after exposure to γ-irradiation. In addition, its labelling property caused the polymerase extension to stop in the 5-formyluracil site, which gave us more information than did the fluorescence itself. The idea of detecting 5-formyluracil might be used in the synthesis of other modified diaminofluoresceins.

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Publication details

The article was received on 10 Feb 2017, accepted on 02 Apr 2017 and first published on 05 Apr 2017


Article type: Edge Article
DOI: 10.1039/C7SC00637C
Citation: Chem. Sci., 2017, Accepted Manuscript
  • Open access: Creative Commons BY license
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    Both enrichment and fluorogenic labelling of 5-formyluracil in DNA

    X. Zhou, C. Liu, Y. Wang, X. Zhang, F. Wu, W. Yang, G. Zhou, Q. Yao, J. Wang, Y. Chen and S. Wang, Chem. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7SC00637C

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