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Issue 6, 2017
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An N-nitrosation reactivity-based two-photon fluorescent probe for the specific in situ detection of nitric oxide

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Abstract

In situ fluorescence imaging of nitric oxide (NO) is a powerful tool for studying the critical roles of NO in biological events. However, the selective imaging of NO is still a challenge because most currently available fluorescent probes rely on the o-phenylenediamine (OPD) recognition site, which reacts with both NO and some abundant reactive carbonyl species (RCS) (such as dehydroascorbic acid and methylglyoxal) and some reactive oxygen/nitrogen species (ROS/RNS). To address this problem, a new fluorescent probe, NCNO, based on the N-nitrosation of aromatic secondary amine was designed to bypass the RCS, ROS, and RNS interference. As was expected, the probe NCNO could recognize NO with pronounced selectivity and sensitivity among ROS, RNS, and RCS. The probe was validated by detecting NO in live cells and deep tissues owing to its two-photon excitation and red-light emission. It was, hence, applied to monitor NO in ischemia reperfusion injury (IRI) in mice kidneys by two-photon microscopy for the first time, and the results vividly revealed the profile of NO generation in situ during the renal IRI process.

Graphical abstract: An N-nitrosation reactivity-based two-photon fluorescent probe for the specific in situ detection of nitric oxide

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Publication details

The article was received on 26 Jan 2017, accepted on 14 Apr 2017 and first published on 20 Apr 2017


Article type: Edge Article
DOI: 10.1039/C7SC00416H
Citation: Chem. Sci., 2017,8, 4533-4538
  • Open access: Creative Commons BY-NC license
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    An N-nitrosation reactivity-based two-photon fluorescent probe for the specific in situ detection of nitric oxide

    Z. Mao, H. Jiang, Z. Li, C. Zhong, W. Zhang and Z. Liu, Chem. Sci., 2017, 8, 4533
    DOI: 10.1039/C7SC00416H

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