Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Dual-targeted peptide-conjugated multifunctional fluorescent probe with AIEgen for efficient nucleus-specific imaging and long-term tracing of cancer cells

Abstract

Precisely targeted transportation of long-term tracing regent to nucleus with low toxicity is one of the most challenging concerns in revealing the cancer cell behaviours. Here, we report a dual-targeted peptide-conjugated multifunctional fluorescent probe (RGD-NLS-CPP-cNGR-PyTPE, TNCTP) with aggregation-induced emission (AIE) characteristic, for efficient nucleus-specific imaging, long-term and low-toxicity tracing of cancer cells. TNCTP mainly consists of two components: one is the functionalized combinatorial peptides (TNCT) contains two targeted peptide (RGD and cNGR), a nuclear localization signal (NLS) and a cell penetrating peptide (CPP), which can specific bind to cell surface and effective enter into nucleus; the other one is the AIE-active tetraphenylethene derivative (PyTPE, a typical AIEgen) as fluorescence imaging reagent. In the presence of integrin αvβ3 and aminopeptidase N (CD13), TNCTP can specific bind to both of them using RGD and cNGR, respectively, lighting up its yellow fluorescence. Because of CPP, TNCTP can be effectively integrated into the cytoplasm, and then be delivered into nucleus with the help of NLS. TNCTP exhibited strong fluorescence in nucleus of the integrin αvβ3 and CD13 overexpression cells due to the specific-targeting ability, efficient transport capacity and aggregation induced emission characteristic in more crowded space. Furthermore, TNCTP can be applied for long-term tracing in living cells and scarcely affect the normal cells with negligible toxicity more than ten times passages.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 26 Jan 2017, accepted on 19 Apr 2017 and first published on 19 Apr 2017


Article type: Edge Article
DOI: 10.1039/C7SC00402H
Citation: Chem. Sci., 2017, Accepted Manuscript
  • Open access: Creative Commons BY license
  •   Request permissions

    Dual-targeted peptide-conjugated multifunctional fluorescent probe with AIEgen for efficient nucleus-specific imaging and long-term tracing of cancer cells

    Y. Cheng, C. Sun, X. Ou, B. Liu, X. Lou and F. Xia, Chem. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7SC00402H

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author