Jump to main content
Jump to site search


A flow cytometry assay to quantify intercellular exchange of membrane components

Author affiliations

Abstract

Membrane-compound exchange is vital for cell-to-cell communication, yet quantification of this process is difficult. Here we present a method using flow cytometry in combination with bioorthogonal and fluorescent labelling techniques to quantify the amount of exchange of cholesterol and sialylated compounds between cells. We demonstrate that direct cell–cell contact is the likely mechanism of sterol-exchange and show that by manipulating the contact time between cells using complementary coiled-coil peptides results in an enhanced exchange rate of membrane components between cells.

Graphical abstract: A flow cytometry assay to quantify intercellular exchange of membrane components

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Jan 2017, accepted on 20 May 2017 and first published on 24 May 2017


Article type: Edge Article
DOI: 10.1039/C7SC00260B
Citation: Chem. Sci., 2017, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    A flow cytometry assay to quantify intercellular exchange of membrane components

    D. Poulcharidis, K. Belfor, A. Kros and S. I. van Kasteren, Chem. Sci., 2017, Advance Article , DOI: 10.1039/C7SC00260B

Search articles by author

Spotlight

Advertisements