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Issue 8, 2017
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A flow cytometry assay to quantify intercellular exchange of membrane components

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Abstract

Membrane-compound exchange is vital for cell-to-cell communication, yet quantification of this process is difficult. Here we present a method using flow cytometry in combination with bioorthogonal and fluorescent labelling techniques to quantify the amount of exchange of cholesterol and sialylated compounds between cells. We demonstrate that direct cell–cell contact is the likely mechanism of sterol-exchange and show that by manipulating the contact time between cells using complementary coiled-coil peptides results in an enhanced exchange rate of membrane components between cells.

Graphical abstract: A flow cytometry assay to quantify intercellular exchange of membrane components

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Publication details

The article was received on 18 Jan 2017, accepted on 20 May 2017 and first published on 24 May 2017


Article type: Edge Article
DOI: 10.1039/C7SC00260B
Citation: Chem. Sci., 2017,8, 5585-5590
  • Open access: Creative Commons BY-NC license
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    A flow cytometry assay to quantify intercellular exchange of membrane components

    D. Poulcharidis, K. Belfor, A. Kros and S. I. van Kasteren, Chem. Sci., 2017, 8, 5585
    DOI: 10.1039/C7SC00260B

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