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Issue 41, 2017, Issue in Progress
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Benzene-glycol nucleic acid (BGNA)–DNA chimeras: synthesis, binding properties, and ability to elicit human RNase H activity

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Abstract

This paper describes the synthesis and properties of benzene-glycol nucleic acid (BGNA)–DNA chimeras containing four nucleoside analogs – thymidine, cytidine, adenosine, and guanosine – with a base-benzene-glycol structure. We found that the BGNA–DNA chimeras are able to form thermally and thermodynamically stable duplexes with complementary RNAs, and have base-discriminating abilities. The BGNA–DNA chimeras were 20-fold more stable in a buffer containing 30% bovine serum than unmodified DNA. Furthermore, BGNA–DNA chimera/RNA duplexes were found to be good substrates for human RNase H. Thus, BGNA–DNA chimeras are good candidates for the development of therapeutic antisense molecules.

Graphical abstract: Benzene-glycol nucleic acid (BGNA)–DNA chimeras: synthesis, binding properties, and ability to elicit human RNase H activity

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Publication details

The article was received on 06 Apr 2017, accepted on 28 Apr 2017 and first published on 11 May 2017


Article type: Paper
DOI: 10.1039/C7RA03896H
Citation: RSC Adv., 2017,7, 25378-25386
  • Open access: Creative Commons BY license
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    Benzene-glycol nucleic acid (BGNA)–DNA chimeras: synthesis, binding properties, and ability to elicit human RNase H activity

    N. Niwa, S. Shimizu, Y. Maeda, H. Hiroak and Y. Ueno, RSC Adv., 2017, 7, 25378
    DOI: 10.1039/C7RA03896H

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