Issue 52, 2017, Issue in Progress

Nanoparticles co-delivering pVSVMP and pIL12 for synergistic gene therapy of colon cancer

Abstract

Nanoparticles delivering therapeutic genes have promising applications in cancer treatments. Here, we used biodegradable heparin–polyethyleneimine (HPEI) nanoparticles to co-deliver two genes (vesicular stomatitis virus matrix protein, VSVMP; interleukin-12, IL12) for cancer therapy. These HPEI nanoparticles can bind the plasmid expressing VSVMP (pVSVMP) and the plasmid expressing IL12 (pIL12) through electrostatic interactions, and mediate the simultaneous expression of VSVMP and IL12 in colon cancer cells in vitro and in vivo. After intraperitoneal administration, the HPEI/pIL12 + pVSVMP complexes efficiently inhibit the growth of intraperitoneal metastasis of C-26 colon cancer, showing synergistic anticancer efficacy, without obvious systemic adverse effects. While VSVMP induces apoptosis, VSVMP and IL12 synergistically generate an anticancer immune response, which renders the potent anticancer effect of HPEI/pIL12 + pVSVMP. Our data suggest that VSVMP and IL12 could synergistically inhibit cancer survival in vivo, and the HPEI nanoparticle co-delivered VSVMP and IL12 might have potential application in treating intraperitoneal metastasis of colon cancer.

Graphical abstract: Nanoparticles co-delivering pVSVMP and pIL12 for synergistic gene therapy of colon cancer

Article information

Article type
Paper
Submitted
31 Mar 2017
Accepted
02 Jun 2017
First published
28 Jun 2017
This article is Open Access
Creative Commons BY license

RSC Adv., 2017,7, 32613-32623

Nanoparticles co-delivering pVSVMP and pIL12 for synergistic gene therapy of colon cancer

Y. Xiao, Y. Yang, Y. Wu, C. Wang, H. Cheng, W. Zhao, Y. Li, B. Liu, J. Long, W. Guo, G. Gao and M. Gou, RSC Adv., 2017, 7, 32613 DOI: 10.1039/C7RA03727A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

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