Jump to main content
Jump to site search

Issue 8, 2017, Issue in Progress
Previous Article Next Article

A novel microfluidic device to model the human proximal tubule and glomerulus

Author affiliations

Abstract

We have developed a re-usable multi-layer microfluidic device to model the human kidney that incorporates a porous growth substrate, physiological fluid flow, and the passive filtration of the glomerulus. The target cell line in this project is HK-2, immortalized human kidney proximal tubule cells. Cells were exposed to a shear stress of 0.8 dyne cm−2 within the device and monitored for protein expression, cytoskeletal reorganization, and increased molecular transport. Additionally, an endothelial cell-seeded glomerular filter was added to allow for more realistic “primary urine” within these devices. The results of this research suggest that cells grown within this microfluidic device exhibit more in vivo-like behaviour than those grown using traditional culturing methods, and that the filtration of serum proteins by the glomerulus is necessary for healthy cell function. The addition of this glomerulus-mimic to the device provides the first in vitro model of passive glomerular filtration coupled with the proximal tubule.

Graphical abstract: A novel microfluidic device to model the human proximal tubule and glomerulus

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Oct 2016, accepted on 19 Dec 2016 and first published on 16 Jan 2017


Article type: Paper
DOI: 10.1039/C6RA25641D
Citation: RSC Adv., 2017,7, 4216-4225
  • Open access: Creative Commons BY license
  •   Request permissions

    A novel microfluidic device to model the human proximal tubule and glomerulus

    Courtney M. Sakolish and G. J. Mahler, RSC Adv., 2017, 7, 4216
    DOI: 10.1039/C6RA25641D

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements