Jump to main content
Jump to site search

Issue 6, 2017
Previous Article Next Article

Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Author affiliations

Abstract

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.

Graphical abstract: Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Aug 2016, accepted on 13 Feb 2017 and first published on 17 Feb 2017


Article type: Research Article
DOI: 10.1039/C6QO00448B
Citation: Org. Chem. Front., 2017,4, 1058-1062
  • Open access: Creative Commons BY license
  •   Request permissions

    Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

    X. Sun, M. L. Odyniec, A. C. Sedgwick, K. Lacina, S. Xu, T. Qiang, S. D. Bull, F. Marken and T. D. James, Org. Chem. Front., 2017, 4, 1058
    DOI: 10.1039/C6QO00448B

    This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements