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Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

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Abstract

Based on the complexation of phenylboronic acid (PBA) with Alizarin Red S (ARS), we developed a new chemosensor for the detection of hydrogen peroxide (H2O2) in aqueous media. This platform has demonstrated its ability to detect H2O2via colorimetric, fluorometric, and electrochemical measurements. The experimental observations reveal that the system displays a red-shifted visible colour change, on–off fluorescence response indicating release of indicator (ARS) and turn-on electrochemical signal indicating generation of phenol, after reaction with H2O2. With this work we have demonstrated that our reaction-based indicator displacement assay (RIA) systems, can be employed as an assay for H2O2 and hydrogen peroxide-related species for environmental and physiological detection.

Graphical abstract: Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

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Publication details

The article was received on 08 Aug 2016, accepted on 13 Feb 2017 and first published on 17 Feb 2017


Article type: Research Article
DOI: 10.1039/C6QO00448B
Citation: Org. Chem. Front., 2017, Advance Article
  • Open access: Creative Commons BY license
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    Reaction-based indicator displacement assay (RIA) for the colorimetric and fluorometric detection of hydrogen peroxide

    X. Sun, M. L. Odyniec, A. C. Sedgwick, K. Lacina, S. Xu, T. Qiang, S. D. Bull, F. Marken and T. D. James, Org. Chem. Front., 2017, Advance Article , DOI: 10.1039/C6QO00448B

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