Supramolecular Conjugated Polymer Materials for Organelle Imaging in Living Cells
A novel supramolecular system based on conjugated polymers was developed to realize specific targeting and imaging of organelles in living cells. Carboxyl group-modified polythiophene derivative (PT) forms complex with six histidines equipped Tat peptide via Ni2+ ion chelation, hydrogen bonds and electrostatic interactions (PT/Ni2+/Tat-His6). The complex can form reassembly with RFP-His10 due to the relatively high affinity of Ni2+ towards His10-tag compared to His6-tag. Thus, PT/Ni2+/Tat-His6 complex could selectively label and image organelles expressed with His10-tag in living cells with high stability.