Jump to main content
Jump to site search

Issue 48, 2017
Previous Article Next Article

Self-assembled core–shell-corona multifunctional non-viral vector with AIE property for efficient hepatocyte-targeting gene delivery

Author affiliations

Abstract

With the aim to develop a novel multifunctional gene delivery system that may overcome the common barriers of gene transfection, two amphiphilic molecules with tetraphenyl ethylene (TPE) as a hydrophobic moiety and linear PEI-galactose as a hydrophilic part were elaborately designed and synthesized. The molecules could self-assemble to form micelles and exhibited aggregation-induced emission (AIE) property, which may be used for cell imaging. Several assays revealed that the disulfide bond in TSPG may facilitate vector decomposition and DNA release, resulting in higher transfection efficiency and lower cytotoxicity than TCPG and PEI 25 kDa. These vectors showed excellent serum tolerance, and even higher efficiency could be obtained in transfection with the presence of serum. Moreover, the transfection in different cell lines showed that TSPG complex could induce higher gene expression in asialoglycoprotein receptor-rich HepG2 cells than in HeLa cells, which lack such receptors. Galactose competition assay also demonstrated that the galactose moiety could afford the vector with targeting ability toward hepatoma cells. Cellular uptake inhibition assay indicated that the DNA complex entered the cell mainly via caveolae-mediated endocytosis. Results showed that TSPG may serve as a promising reagent for simultaneous gene transfection and imaging with good biocompatibility and cell targeting ability.

Graphical abstract: Self-assembled core–shell-corona multifunctional non-viral vector with AIE property for efficient hepatocyte-targeting gene delivery

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Sep 2017, accepted on 30 Oct 2017 and first published on 10 Nov 2017


Article type: Paper
DOI: 10.1039/C7PY01520H
Citation: Polym. Chem., 2017,8, 7486-7498
  •   Request permissions

    Self-assembled core–shell-corona multifunctional non-viral vector with AIE property for efficient hepatocyte-targeting gene delivery

    B. Wang, P. Chen, J. Zhang, X. Chen, Y. Liu, Z. Huang, Q. Yu, J. Zhang, W. Zhang, X. Wei and X. Yu, Polym. Chem., 2017, 8, 7486
    DOI: 10.1039/C7PY01520H

Search articles by author

Spotlight

Advertisements