Jump to main content
Jump to site search

Issue 42, 2017
Previous Article Next Article

Photochromic RAFT reagent helps construct superior photoswitchable fluorescent polymer nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging

Author affiliations

Abstract

Superior photoswitchable fluorescent polymer nanoparticles (PFPNs) are highly desirable for photo-rewritable data storage and ultrahigh-resolution bioimaging. Herein, 2-(3′,3′-dimethyl-6-nitrospiro[chromene-2,2′-indolin]-1′-yl)ethyl 2-(((dodecylthio)-carbonothioyl)thio)-2-methylpropanoate (SPTTC), a novel photochromic spiropyran-based reversible addition–fragmentation chain transfer (RAFT) reagent, and 5-dimethylaminonaphthalene-N-allyl-1-sulfonamide (DNS), the energy-level matched green fluorescent dye, were firstly synthesized. Then, they are used as donor and acceptor of the designed fluorescence resonance energy transfer (FRET) system to help fabricate superior dual-color PFPNs via a simple one-pot RAFT miniemulsion method. Thanks to its multiple merits such as the photochromic property, improved miscibility, and the ability to control polymerization rate, not only did the designed SPTTC provide the PFPNs with superior photoswitchable fluorescence properties such as high energy transfer efficiency (up to 91%), high dual-color contrast (green and red), rapid photoresponsiveness (2–4 min) and outstanding photoreversibility upon irradiation with UV and visible light, but it also endowed PFPNs with controllable molecular weight and narrow polydispersity index (PDI), excellent cell membrane permeability and long-term fluorescence stability. Moreover, the as-prepared PFPNs were successfully used for rewritable fluorescence patterning and intracellular dual-color imaging due to its prominent properties.

Graphical abstract: Photochromic RAFT reagent helps construct superior photoswitchable fluorescent polymer nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Aug 2017, accepted on 02 Oct 2017 and first published on 03 Oct 2017


Article type: Paper
DOI: 10.1039/C7PY01408B
Citation: Polym. Chem., 2017,8, 6520-6526
  •   Request permissions

    Photochromic RAFT reagent helps construct superior photoswitchable fluorescent polymer nanoparticles for rewritable fluorescence patterning and intracellular dual-color imaging

    J. Chen, W. Zhong, M. Xue, H. Wang, M. Yu, P. Zhang and P. Yi, Polym. Chem., 2017, 8, 6520
    DOI: 10.1039/C7PY01408B

Search articles by author

Spotlight

Advertisements