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Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

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Abstract

The development of an effective method for imaging of lysosomal hypochlorous acid (HClO) is of great significance to better understand the roles of HClO-related diseases. Herein, we report on the fabrication of fluorescence resonance energy transfer (FRET)-based fluorescent polymer dots (FPD) for the ratiometric imaging of lysosomal HClO in living cells via a simple co-precipitation method. This FRET-based construction strategy takes the advantage of flexible design by incorporating target-inert fluorogen (donor) and target-sensitive fluorogen (acceptor) into the nanoparticle to avoid leakage and photobleaching problems. The as-prepared FPD exhibits good water dispersibility, fast response (<30 s), high sensitivity (∼5.1 nM), outstanding selectivity, excellent photostability and long-term stability (>15 weeks). Moreover, the resultant FPD with good membrane permeability could mainly locate in the lysosomes and act as a probe for the ratiometric imaging of lysosomal HClO in living HeLa cells.

Graphical abstract: Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

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Publication details

The article was received on 01 Aug 2017, accepted on 29 Aug 2017 and first published on 29 Aug 2017


Article type: Paper
DOI: 10.1039/C7PY01289F
Citation: Polym. Chem., 2017, Advance Article
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    Ratiometric imaging of lysosomal hypochlorous acid enabled by FRET-based polymer dots

    H. Wang, P. Zhang, Y. Hong, B. Zhao, P. Yi and J. Chen, Polym. Chem., 2017, Advance Article , DOI: 10.1039/C7PY01289F

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