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Bio-inspired peptide decorated dendrimers for a robust antibacterial coating on hydroxyapatite

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Abstract

Bacterial colonization on implanted biomaterials remains a clinically significant problem. In order to achieve relatively long-term antibacterial activity and reduce the incidence of infections associated with the use of biomaterials, a salivary statherin protein (SSP) inspired poly(amidoamine) dendrimer (SSP-PAMAM-NH2) was synthesized and characterized. PAMAM-NH2 has numerous peripheral amino groups, and thus possesses effective antibacterial activity. The SSP bio-inspired peptide sequence DDDEEKC was conjugated to PAMAM-NH2 since it has a strong capability of adsorbing on hydroxyapatite (HA). Moreover, SSP-PAMAM-NH2 is a zwitterionic polymer possessing cationic amino groups and anionic carboxylic groups, thus it can form aggregates by intermolecular electrostatic interactions, thereby promoting its adsorption on HA. Adsorption tests by ATR-IR, UV, QCM-D, and CLSM, all indicated that SSP-PAMAM-NH2 can tightly adsorb on the HA surface. We found that even after being incubated in PBS for 4 weeks, the SSP-PAMAM-NH2 treated HA disks still retained stable antibacterial activity, while the inhibitory impact of PAMAM-NH2 treated disks had disappeared. Animal experiments also demonstrated that SSP-PAMAM-NH2 could significantly reduce infection of HA implanted into the medullary cavity of rats.

Graphical abstract: Bio-inspired peptide decorated dendrimers for a robust antibacterial coating on hydroxyapatite

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Publication details

The article was received on 16 May 2017, accepted on 21 Jun 2017 and first published on 23 Jun 2017


Article type: Paper
DOI: 10.1039/C7PY00811B
Citation: Polym. Chem., 2017, Advance Article
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    Bio-inspired peptide decorated dendrimers for a robust antibacterial coating on hydroxyapatite

    Y. Gou, X. Yang, L. He, X. Xu, Y. Liu, Y. Liu, Y. Gao, Q. Huang, K. Liang, C. Ding, J. Li, C. Zhao and J. Li, Polym. Chem., 2017, Advance Article , DOI: 10.1039/C7PY00811B

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