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The SOS Chromotest applied for screening plant antigenotoxic agents against ultraviolet radiation

Abstract

In this work, we investigated the usefulness of the SOS Chromotest for screening plant antigenotoxic agents against ultraviolet radiation (UV). Fifty Colombian plant extracts obtained by supercritical fluid (CO2) extraction, twelve plant extract constituents (apigenin, carvacrol, β-caryophyllene, 1,8-cineole, citral, p-cymene, geraniol, naringenin, pinocembrin, quercetin, squalene, and thymol) and five standard antioxidant and/or photoprotective agents (curcumin, epigallocatechin gallate, resveratrol, α-tocopherol, and Trolox®) were evaluated for their genotoxicity and antigenotoxicity against UV using the SOS Chromotest. None of the plant extracts, constituents or agents were genotoxic in the SOS Chromotest at tested concentrations. Based on minimal extract concentration that significantly inhibited UV-genotoxicity (CIG), five plant extracts were antigenotoxic against UV as follows: Baccharis nítida (16 µg/mL) = Solanum crotonifolium (16 µg/mL) > Hyptis suaveolens (31 µg/mL) = Persea caerulea (31 µg/mL) > Lippia origanoides (62 µg/mL). Based on CIG values, the flavonoid compounds showed the highest antigenotoxic potential as follows: apigenin (7 µM) > pinocembrin (15 µM) > quercetin (26 µM) > naringenin (38 µM) > epigallocatechin gallate (108 µM) > resveratrol (642 µM). UV-genotoxicity inhibition with epigallocatechin gallate, naringenin and resveratrol was related to their capability for inhibit protein synthesis. A correlation analysis between compound antigenotoxicity estimates and antioxidant activity evaluated by oxygen radical absorbance capacity (ORAC) assay showed that these activities were not related. The usefulness of SOS Chromotest for bioprospecting of plant antigenotoxic agents against UV was discussed.

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Publication details

The article was received on 23 Jan 2017, accepted on 05 Jul 2017 and first published on 13 Jul 2017


Article type: Paper
DOI: 10.1039/C7PP00024C
Citation: Photochem. Photobiol. Sci., 2017, Accepted Manuscript
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    The SOS Chromotest applied for screening plant antigenotoxic agents against ultraviolet radiation

    J. L. Fuentes, A. García-Forero, N. Quintero Ruiz, C. A. Prada Medina, N. Rey-Castellano, D. A. Franco-Niño, D. A. Contreras-García , Y. Cordoba-Campo and E. Stashenko, Photochem. Photobiol. Sci., 2017, Accepted Manuscript , DOI: 10.1039/C7PP00024C

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