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Issue 7, 2017
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Light induced cytotoxicity of nitrofurantoin toward murine melanoma

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Abstract

The cytotoxicity of nitrofurantoin (NFT) in the dark and after light exposure (UVA irradiation, λ = 385 nm) was evaluated in murine melanoma B16F10 cells. NFT induces both cell proliferation and inhibition of cell viability. The dominance of one or the other effect depends on the drug concentration, incubation time (tinc) and irradiation dose. The uptake of NFT in these cells, as well as its photocytotoxicity, reaches saturation after 24 hours of incubation. The mechanism of cell death in the dark is associated with the enzymatic release of nitric oxide (NO). The increase of NFT cytotoxicity under light irradiation is associated with the increase of NO concentration due to photorelease. NO photorelease by NFT in solution was confirmed by chemiluminescence, while NO formation in cells was confirmed by fluorescence microscopy using DAF-2DA, a specific indicator of NO in living cells. The NFT does not enter nuclei, distributing preferentially in the cell cytoplasm, as shown by fluorescence microscopy.

Graphical abstract: Light induced cytotoxicity of nitrofurantoin toward murine melanoma

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Publication details

The article was received on 28 Jan 2017, accepted on 21 Apr 2017 and first published on 24 Apr 2017


Article type: Paper
DOI: 10.1039/C6PP00306K
Citation: Photochem. Photobiol. Sci., 2017,16, 1071-1078
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    Light induced cytotoxicity of nitrofurantoin toward murine melanoma

    L. P. Ferreira, G. G. Parra, D. C. K. Codognato, A. M. Amado and R. S. da Silva, Photochem. Photobiol. Sci., 2017, 16, 1071
    DOI: 10.1039/C6PP00306K

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