Jump to main content
Jump to site search


Hydroxylation of anilides by engineered cytochrome P450BM3

Author affiliations

Abstract

Biocatalytic direct monohydroxylation of anilides has been achieved on preparative scale using mutant cytochrome P450BM3 enzymes. Representative mono- and disubstituted N-trifluoromethanesulfonyl anilides are shown to be converted in most cases to the corresponding 4-hydroxy derivatives, with substituent hydroxylation also occurring in two cases. By mutation variation, it is possible to achieve selective hydroxylation of either ring- or side-chain sites.

Graphical abstract: Hydroxylation of anilides by engineered cytochrome P450BM3

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2017, accepted on 03 Oct 2017 and first published on 03 Oct 2017


Article type: Paper
DOI: 10.1039/C7OB02236K
Citation: Org. Biomol. Chem., 2017, Advance Article
  •   Request permissions

    Hydroxylation of anilides by engineered cytochrome P450BM3

    J. A. O'Hanlon, X. Ren, M. Morris, L. L. Wong and J. Robertson, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB02236K

Search articles by author

Spotlight

Advertisements