Issue 37, 2017

A fluorescent pH probe for acidic organelles in living cells

Abstract

A water-soluble pH sensor, 2-(6-(4-aminostyryl)-1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)-N, N-dimethylethanamine (ADA), was synthesized based on the molecular design of photoinduced electron transfer (PET) and intramolecular charge transfer (ICT). The fluorescence emission response against a pH value is in the range 3–6, which is suitable for labelling intracellular pH-dependent microenvironments. After biological evolution, ADA is more than a pH biosensor because it is also an endocytosis pathway tracking biosensor that labels endosomes, late endosomes, and lysosome pH gradients. From this, the emissive aggregates of ADA and protonated-ADA in these organs were evaluated to explore how this probe stresses emission colour change to cause these unique cellular images.

Graphical abstract: A fluorescent pH probe for acidic organelles in living cells

Supplementary files

Article information

Article type
Paper
Submitted
16 Aug 2017
Accepted
04 Sep 2017
First published
04 Sep 2017

Org. Biomol. Chem., 2017,15, 7936-7943

A fluorescent pH probe for acidic organelles in living cells

J. Chen, C. Chen and C. Chang, Org. Biomol. Chem., 2017, 15, 7936 DOI: 10.1039/C7OB02037F

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