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A fluorescent pH probe for acidic organelles in living cells

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Abstract

A water-soluble pH sensor, 2-(6-(4-aminostyryl)-1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)-N, N-dimethylethanamine (ADA), was synthesized based on the molecular design of photoinduced electron transfer (PET) and intramolecular charge transfer (ICT). The fluorescence emission response against a pH value is in the range 3–6, which is suitable for labelling intracellular pH-dependent microenvironments. After biological evolution, ADA is more than a pH biosensor because it is also an endocytosis pathway tracking biosensor that labels endosomes, late endosomes, and lysosome pH gradients. From this, the emissive aggregates of ADA and protonated-ADA in these organs were evaluated to explore how this probe stresses emission colour change to cause these unique cellular images.

Graphical abstract: A fluorescent pH probe for acidic organelles in living cells

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Publication details

The article was received on 16 Aug 2017, accepted on 04 Sep 2017 and first published on 04 Sep 2017


Article type: Paper
DOI: 10.1039/C7OB02037F
Citation: Org. Biomol. Chem., 2017, Advance Article
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    A fluorescent pH probe for acidic organelles in living cells

    J. Chen, C. Chen and C. Chang, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB02037F

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